Other University of Arizona Imaging Facilities
There are a number of fee-for-service facilities on the UA campus. Many are part of the Biotechnology Division of the Arizona Research Laboratories. As an example, ARL-Biotech supports a number of campus-wide service facilities such as; biological computing, genomics, flow cytometry, proteomics, imaging/microscopy, and magnetic resonance. Other service facilities are administered within specific Colleges, Departments, or Centers of Excellence (Cancer Center, SWEHSC, etc).
Message from Doug Cromey, Manager of Confocal Microscopy Facility: 520-626-2824, cromey@arizona.edu
The table below lists only the facilities that are in some way related to imaging, since they are the ones that Doug Cromey is most familiar with. This table lists those facilities that are open to the entire campus community.
Tissue preparation and staining for light microscopy |
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Andrea Grantham (626-4415) |
AHSC 4212 |
Routine paraffin (embedding, sectioning & staining) and frozen section (sectioning & staining) histology and special stains. |
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Kathy McDaniel (626-2448) |
AZCC 3959 |
Routine paraffin and frozen section histology. Tissue microarrays. Automated immunohistochemical staining with over 80 human and mouse antibodies. |
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DMetrix microscope slide scanner |
Lynne Richter (626-2796) |
AHSC 5210 |
Can scan an entire 1x3 glass microscope slide in color at 20x. Exports digital images of the scans in a variety of file formats and magnifications. |
Microscope with color CCD camera for routine light microscopy imaging? |
There is a microscope like this at the University Spectroscopy & Imaging Facilities on the main campus (see below). There is also a lower resolution color camera (710x512) on an image analysis workstation in the AZCC (no support contact). For greyscale images, see the CBA Image Acquisition & Analysis workstation (below). Individual labs and departments may have this type of equipment, ask around. |
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Fluorescence-related |
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Doug Cromey (626-2824) |
LSN 410 |
Zeiss LSM 510Meta-NLO multiphoton confocal microscope. |
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Carl Boswell (954-7053) |
AZCC 0953 |
Nikon PCM2000 confocal, 3 color imaging of fixed samples. |
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Barb Carolus (621-2047) |
AZCC |
Full service flow cytometry and cell sorting. If you are interested in quantitating fluorescence intensity, flow is the way to go. |
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Intravital microscope |
Urs Utzinger |
Keating |
Currently being built in BME. Microscope stage will accommodate small animals for microscopic imaging of organs and tissues. Confocal and multiphoton, as well as fluorescence lifetime imaging. |
Brenda Bagget |
MRB 130 & BRL |
Whole animal imaging using internal fluorescent probes and high-sensitivity camera. Part of SWAIR (see below). |
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David Elliott (626-7870) |
MRB 330M |
Deconvolution fluorescence microscope. Ideal for low-light samples. Has capability for time-lapse imaging of live cells. |
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Electron Microscopy, AFM and SPM |
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Tony Day (626-6009) |
LSN 410 |
Biological transmission and scanning electron microscopy. Tissue processing, sectioning and microscope operation. |
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Paul Lee |
Chemical Sci 135 |
Atomic force and Scanning probe microscopes. |
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David Bentley (biological) Gary Chandler (materials) |
MARLEY Suite 101 |
Transmission & Scanning electron microscopy facility, both biological & materials sciences expertise available. |
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Computerized Image Analysis |
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Doug Cromey (626-2824) |
LSN 429 |
Inverted microscope with multiple imaging modalities and 10bit greyscale CCD camera. Can perform time-lapse imaging on live cells with this instrument. SimplePCI Image analysis software can be used for counting, measuring, and analyzing images from any source. Users perfom their own analysis with assistance from Doug Cromey in setting up the semi-automated software macros. Note, identical image analysis software exists on a workstation in the AZCC, however, there is no designated support contact to assist users. |
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Marvin Landis |
Campus Computer Center |
3D reconstruction and visualization. Immersive 3D environment. |
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Radiology imaging |
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See website for contacts |
Part of ARIBI. Includes Single Photon Emission Computed Tomography, Biological Magnetic Resonance, Optical Coherence Tomography, and in vivo bioluminescence (see above) |
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